Vuotto, C., Barbanti, F., Mastrantonio, P., & Donelli, G. (2013)


Objectives: To evaluate the ability of the probiotic strain L actobacillus brevis CD 2 to inhibit the opportunistic anaerobe P revotella melaninogenica (PM 1), a well‐known causative agent of periodontitis.

Materials and methods: The inhibitory effect of L actobacillus CD 2 on P revotella PM 1 biofilm was assessed both by exposing the anaerobe to the supernatant of the probiotic strain and by growing the two strains to obtain single or mixed biofilms. The inhibitory effect of CD 2 on PM 1 was also checked by the agar overlay method.

Results: The development of PM 1 biofilm was strongly affected (56% decrease in OD value) by the CD 2 supernatant after 96 h. A dose‐dependent biofilm reduction was also observed at 1/10 and 1/100 dilutions of supernatant. Confocal microscopy on the mixed biofilms revealed the ability of CD 2 to prevail on PM 1, greatly reducing the biofilm of the latter.

Conclusion: It has been hypothesized a multifactorial nature of the inhibition mechanism, the strong adherence ability of CD 2 strain together with the released metabolites presumably contributing to the reduction in the PM 1 biofilm detected by confocal microscopy

Oral Diseases, 20(7), 668–674